Application

Research CategoryApoptosis & Cancer

Western blot: 0.5 to 1 µg/mL.

MOLT4 cell lysate can be used as positive control and an approximate 17 kDa band should be detected.

Optimal working dilutions must be determined by end user.

Anti-Survivin Antibody, N-terminus detects level of Survivin & has been published & validated for use in WB.

Research Sub CategoryBCL2 & Inhibition

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rabbit anti-Survivin (N-terminal) polyclonal antibody was raised against a peptide corresponding to amino acids 1 to 12 of human origin (Ambrosini et al. 1997).

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Format: Purified

Specificity

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4) and TIAP1-3. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas (Ambrosini et al. 1997).

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.